CST Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 9664S

货号： 9664S
品牌： CST

规格：100ul

使用与人 caspase-3 Asp175 周围的氨基末端残基相对应的合成肽对动物进行免疫接种来产生单克隆抗体。

Immunoprecipitation of extracts from Jurkat cells, untreated or etoposide-treated (25uM, 5hrs), using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb. Western blot was performed using the same antibody.

免疫沉淀分析Jurkat 细胞的提取物，未处理或 etoposide （依托泊苷）(25uM, 5hrs)处理，采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗，然后用同样抗体进行western blot 分析。

Immunohistochemical staining of paraffin-embedded mouse embryo, showing cytoplasmic localization in apoptotic cells, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

免疫组化分析石蜡包埋小鼠胚胎，显示凋亡细胞中裂解的caspase-3定位胞浆，抗体采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with etoposide #2200 (green), using Cleaved Caspase-3(Asp175) (5A1E) Rabbit mAb compared to a nonspecific negative control antibody (red).

Immunohistochemical analysis using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb on SignalSlide (TM) Cleaved Caspase-3 IHC Controls #8104 (paraffin-embedded Jurkat cell, untreated (left) or etoposide-treated (right)).

免疫组化分析，用 SignalSlide (TM) Cleaved Caspase-3 IHC Controls #8104（石蜡包埋的Jurkat 细胞，未处理（左）或 etoposide 处理（右）），采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

Western blot analysis of extracts from C6 (rat), NIH/3T3 (mouse), and Jurkat (human) cells, untreated or treated with staurosporine (1uM, 3hrs) or etoposide (25uM, 5hrs) as indicated, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

Western blot 分析未处理或 staurosporine （星胞菌素） (1uM, 3hrs) 或 etoposide （依托泊苷）(25uM, 5hrs)处理的C6 (大鼠源)、NIH/3T3 (小鼠源)和Jurkat (人源) 细胞。抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb in the presence of control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide (#1050) (right).

免疫组化分析石蜡包埋的小鼠胚胎，抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗，并用对照肽段（左图）或 Cleaved Caspase-3 (Asp175) 封闭肽段 (#1050) 对照(右图)。

Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine treated (right) labeled with Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb#9953 (green). Actin filaments have been labeled with Alexa Fluor^® 555 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析未处理（左）或 Staurosporine (星胞菌素)处理（右）的HT-29 细胞，抗体采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗#9953（绿色）。微丝用Alexa Fluor^® 555 phalloidin（红色）标记。蓝色伪彩=DRAQ5^® #4084 (DNA 荧光染料)。

Immunohistochemical analysis of frozen H1650 xenograft, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

免疫组化分析 H1650 xenograft 冰冻切片，抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。